Journal: Materials Today Bio

Article Title: Human iPSC-EV-loaded nanofiber stent coatings accelerate vascular repair by enhancing EGFR/HIF-1α signaling and suppressing ROCK1-mediated remodeling

doi: 10.1016/j.mtbio.2026.103564

Figure Lengend Snippet: iPSC-EVs modulate hypoxia-adaptive and angiogenic signaling pathways in HUVECs by downregulating negative regulators of the EGFR and HIF-1α signaling axes. HUVECs were cultured under hypoxia and starvation stress conditions and treated with iPSC-EVs or DPBS. (A) RT-qPCR analysis of miRNA target genes. EV treatment resulted in the significant downregulation of ERRFI1 and VHL mRNA levels compared to the DPBS control. (n = 4). (B) Consistent with the suppression of ERRFI1 , EV treatment significantly increased p-EGFR (Tyr1068) levels and the p-EGFR/EGFR ratio while total EGFR protein levels remained unchanged. Additionally, HIF-1α protein significantly accumulated in the EV group, consistent with the downregulation of VHL . (C) Analysis of downstream effectors. The activation of upstream sensors propagated to downstream cascades, evidenced by significantly elevated phosphorylation of ERK1/2 and increased expression of the pro-angiogenic factor VEGF compared to the control. (D) Evaluation of cell proliferation. The EV-treated group exhibited significantly upregulated PCNA protein levels, confirming that the activated signaling pathways resulted in enhanced endothelial proliferation. Data are presented as mean ± SD. n = 4 (A), n = 5 (B, EGFR), and n = 6 (B, HIF-1α, C and D). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Angiogenesis and endothelial markers: HIF-1α (1:1000, A11945, ABclonal), VEGF (1:1000, A23759, ABclonal), and CD31 (1:1000, A18014, ABclonal).

Techniques: Protein-Protein interactions, Cell Culture, Quantitative RT-PCR, Control, Activation Assay, Phospho-proteomics, Expressing

Journal: American Journal of Cancer Research

Article Title: Reactive oxygen species-dependent regulation of hypoxia-inducible factor 1α/C-X-C motif chemokine receptor 4 signaling promotes ozone-induced cancer metastasis

doi: 10.62347/DKDS6537

Figure Lengend Snippet: The effect of ozone exposure on tumor cells was studied by measuring ROS levels in B16F10 melanoma and LL2 lung carcinoma cells. (A) The ROS content was assessed using the ROS-ID Total ROS Detection Kit. Data from the 520 nm fluorescence indicates increased ROS levels. The increase in ROS in (B) B16F10 melanoma and (C) LL2 lung carcinoma cells was observed after three days of treatment with air or ozone, with or without arbutin (1.56 µM). These measurements were performed using the Chekine TM reactive oxygen species detection fluorometric assay. (D) Cell proliferation of B16F10 melanoma and LL2 lung carcinoma was evaluated following three-day ozone exposure, revealing enhanced proliferation in both cell lines. Results are shown as mean ± SD with a sample size of 6; statistical significance is indicated by *P < 0.05, **P < 0.01, and ***P < 0.001. Western blot images display the expression of PCNA and HIF-1α proteins in (E) B16F10 (F) LL2 cells exposed to ozone or air at various time points.

Article Snippet: The HIF-1α inhibitor (SYP-5) was purchased from MedChemExpress (MedChemExpress LLC, Monmouth Junction, NJ, USA).

Techniques: Fluorescence, Western Blot, Expressing

Journal: American Journal of Cancer Research

Article Title: Reactive oxygen species-dependent regulation of hypoxia-inducible factor 1α/C-X-C motif chemokine receptor 4 signaling promotes ozone-induced cancer metastasis

doi: 10.62347/DKDS6537

Figure Lengend Snippet: The effects of ozone on the HIF-1α/CXCR4 pathway and EMT markers in B16F10 and LL2 in vitro models. Western blot images showing levels of HIF-1α, CXCR4, E-cadherin, N-cadherin, MMP-2, and SNAIL proteins. Inset values represent protein expression levels normalized to β-Actin.

Article Snippet: The HIF-1α inhibitor (SYP-5) was purchased from MedChemExpress (MedChemExpress LLC, Monmouth Junction, NJ, USA).

Techniques: In Vitro, Western Blot, Expressing

Journal: American Journal of Cancer Research

Article Title: Reactive oxygen species-dependent regulation of hypoxia-inducible factor 1α/C-X-C motif chemokine receptor 4 signaling promotes ozone-induced cancer metastasis

doi: 10.62347/DKDS6537

Figure Lengend Snippet: The ROS-related effects of in vitro arbutin application on ozone-exposed EMT depolarization and HIF-1α/CXCR4 pathway downregulation in (A) B16F10 and (B) LL2 cells. Protein expressions of HIF-1α, CXCR4, E-cadherin (E-Cad), N-cadherin (N-Cad), MMP-2, and SNAIL are shown with arbutin-reversal treatment. Inset values indicate protein levels normalized to β-Actin. Each experiment was repeated three times with similar results.

Article Snippet: The HIF-1α inhibitor (SYP-5) was purchased from MedChemExpress (MedChemExpress LLC, Monmouth Junction, NJ, USA).

Techniques: In Vitro